TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019

Envelopment analysis for global response to novel 2019 Coronavirus-SARS-COV-2 (COVID-19)

Communities are constantly seeking to manage the damages which are caused by crises. In this regard, health centers have become the most expensive unit of the health system as they provide quick and timely health care services to reduce the effects of unexpected accidents. So, their planning and preparation should be considered as an important part of strategic health policies. The purpose of t...

Experimental Protocol for Detecting SARS-CoV-2 in Screenings and Grit Samples of Wastewater Treatment Plants

Introduction: Although various liquid, solid, and gaseous streams of wastewater treatment plants (WWTPs) have been analyzed in many studies for the presence of SARS-CoV-2 RNA, no study was found to sample and detect SARS-CoV-2 RNA in screenings and grit samples separated from primary treatment units of WWTP. Hence, this study aims to provide an experimental protocol for sampling and extracting ...

TRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies

RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. Although many RNA extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. Many studies have shown that TRIzol is an applicable material for the RNA extracti...

Automated extraction protocol for quantification of SARS-Coronavirus RNA in serum: an evaluation study

BACKGROUND We have previously developed a test for the diagnosis and prognostic assessment of the severe acute respiratory syndrome (SARS) based on the detection of the SARS-coronavirus RNA in serum by real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR). In this study, we evaluated the feasibility of automating the serum RNA extraction procedure in order to increase ...

SARS diagnosis, monitoring and prognostication by SARS-coronavirus RNA detection.